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P14 Intratracheal instillation of air pollution particles in rats is associated with prolonged systemic inflammation, increased leukocyte intracellular F-actin and damage to the vascular endothelium

Adams, Rachel, Al-Bulushi, H, Berube, Kelly Ann and Jones, Timothy Peter 2012. P14 Intratracheal instillation of air pollution particles in rats is associated with prolonged systemic inflammation, increased leukocyte intracellular F-actin and damage to the vascular endothelium. Biorheology 49 (2, 3) , p. 206.

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Abstract

Exposure to particulate air pollution is associated with an increased cardiovascular risk but the mechanisms involved are poorly understood. The aim of this investigation was to assess the effect, in rats, of intratracheal instillation of particulate air pollution on leucocyte activation, intracellular actin polymerisation and damage to the vascular endothelium. Following instillation of air pollution particles blood was collected from controls and 3 days and 6 weeks post instillation (n = 15). Partial differential leucocyte counts were performed. Intracellular F-actin content of blood PMNs and monocytes was determined by staining with FITC-phalloidin and flow cytometry (MFI). Plasma neutrophil elastase, von Willebrand factor (vWF) and sICAM-1 were determined by ELISA. There was a significant increase in monocyte F-actin from 403 ± 41(mean MFI ± SEM) in controls to 606 ± 67 at 3 days post instillation and 484 ± 38 6 days post instillation (p = 0.004, ANOVA) and a decrease in monocyte cell count from 0.193 ± 0.008 ´ 106 cells/ml in controls to 0.150 ± 0.008 ´ 106 cells/ml at 3 days post and 0.149 ± 0.009 ´ 106 cells/ml at 6 weeks post (p = 0.003, ANOVA). There were no significant changes in PMN MFI (p = 0.369, ANOVA) or cell counts (p = 0.753, ANOVA). Plasma neutrophil elastase increased from 175 ± 44 ng/ml in controls to 288 ± 26 ng/ml 3 days post instillation (p=0.038). There was an increase in two markers of endothelial damage. vWF increased from 0.160 ± 0.015 IU/ml in controls to 0.224 ± 0.015 IU/ml at 3 days post and 0.208 ± 0.01 IU/ml at 6 weeks post (p = 0.006, ANOVA). sICAM-1 increased from 17.75 ± 0.70 ng/ml in controls to 19.03 ± 0.33 ng/ml at 3 days post and 21.72 ± 1.16 ng/ml at 6 weeks post (p = 0.009, ANOVA). In conclusion lung exposure caused systemic inflammation, activation and decreased deformability of leukocytes; the effects persisted long after the initial instillation event. Exposure to even short term episodes of high air pollution could increase cardiovascular risk.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Earth and Ocean Sciences
Subjects: Q Science > Q Science (General)
Publisher: IOS Press
ISSN: 0006-355X
Last Modified: 09 Sep 2019 22:08
URI: http://orca.cf.ac.uk/id/eprint/33154

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