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Novel chondroitin sulfate-modified ligands for L-selectin on lymph node high endothelial venules

Derry, Catherine J., Faveeuw, Christelle, Mordsley, Kelly R. and Ager, Ann 1999. Novel chondroitin sulfate-modified ligands for L-selectin on lymph node high endothelial venules. European Journal of Immunology 29 (2) , pp. 419-430. 10.1002/(SICI)1521-4141(199902)29:02<419::AID-IMMU419>3.0.CO;2-A

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Abstract

The migration of lymphocytes into lymph nodes via high endothelial venules (HEV) is dependent on the expression of L-selectin on the lymphocyte cell surface. HEV express several L-selectin ligands including CD34, GlyCAM-1, MAdCAM-1 and two sulfated glycoproteins (Sgp) of 200 kDa and 170 kDa which remain to be identified. In this investigation, labeling with sodium [35S]sulfate, which is incorporated into and forms part of the functional carbohydrate ligand, has been used to isolate and characterize macromolecular L-selectin ligands. High endothelial cells (HEC) cultured from rat lymph node HEV were shown to express ligands for L-selectin. HEC synthesized two groups of sulfated glycoproteins of 150 kDa and > 200 kDa, which were present in conditioned medium. These coeluted on anion exchange chromatography at 1.0 – 1.2 M NaCl and supported calcium-dependent L-selectin-mediated cell adhesion. In common with known L-selectin ligands, Sgp 150 / > 200 were shown to be O-sialoglycoproteins; however, in contrast to other ligands, Sgp 150 / > 200 contained chondroitin sulfate glycosaminoglycan modifications which were required for L-selectin recognition. Chondroitin sulfate-modified ligands for L-selectin were expressed at the HEC surface and by HEV in lymph nodes, suggesting that they may participate in lymphocyte interactions with HEV in vivo.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Subjects: Q Science > QR Microbiology > QR180 Immunology
Uncontrolled Keywords: L-selectin ligand, high endothelial venule, lymphocyte adhesion
Publisher: Wiley-Blackwell
ISSN: 0014-2980
Last Modified: 04 Jun 2017 04:08
URI: http://orca.cf.ac.uk/id/eprint/33337

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