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Stromal architecture and immune tolerance in additive corneal xenografts in rodents

Quantock, Andrew James, Sano, Y., Young, Robert David and Kinoshita, S. 2005. Stromal architecture and immune tolerance in additive corneal xenografts in rodents. Acta Ophthalmologica Scandinavica 83 (4) , pp. 462-466. 10.1111/j.1600-0420.2005.00509.x

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Abstract

Purpose: To examine immune tolerance and corneal ultrastructure following additive corneal xenografts in rodents. Methods: We carried out surgical implantation of excised BALB/c mouse corneal tissue, either freshly isolated (n = 6) or after storage at −20°C for 1 week (n = 7), into the corneas of Wistar rats at approximately mid-stromal depth. Corneal opacity and neovascularization were evaluated postoperatively, and stromal ultrastructure was observed by transmission electron microscopy. Results: Corneal opacification and neovascularization in the weeks after surgery were less prevalent in grafts of frozen-then-thawed tissue than in grafts of fresh tissue. In a well tolerated frozen-then-thawed xenograft, the matrix architecture was normal throughout most of the recipient and donor tissue, but pronounced fibrillar disorganization was evident adjacent to Descemet's membrane. Conclusion: We attribute the improved tolerance of frozen-then-thawed xenografts over fresh xenografts to a reduced cellular immune response.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
Uncontrolled Keywords: cornea; corneal oedema; immune response; xenograft
Publisher: Wiley-Blackwell
ISSN: 1395-3907
Last Modified: 28 Jun 2019 01:59
URI: http://orca.cf.ac.uk/id/eprint/33447

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