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Blotting techniques

White, P. L. and Hibbitts, Samantha Jayne 2005. Blotting techniques. In: Fuchs, J. and Podda, M. eds. Encyclopedia of Medical Genomics and Proteomics, New York: Marcel Dekker, pp. 135-139. (10.3109/9780203997352.028)

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The capacity to separate nucleic acids and proteins by their molecular weight and identify fractions of interest by the hybridization of specific labeled probes is known as blotting. It was originally developed for the analysis of gel‐fractionated DNA and was termed the Southern blot. Northern blotting was soon to follow and permitted the investigation of RNA templates by using an identical procedure. Since then, Western blotting was designed to analyze proteins and a procedure known as Dot/Slot blotting was developed to detect and quantify any nucleic acid sequence without the necessity of processing [restriction fragment length polymorphism (RFLP) and/or separation by electrophoresis] the nucleic acids.

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: genomics, proteomics, gene therapy, viruses, DNA, FISH, microarrays, nucleotides, bacteria
Publisher: Marcel Dekker
ISBN: 9780824755645
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Last Modified: 05 Jun 2017 03:28

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