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Inverse PCR

White, P. L. and Hibbitts, Samantha Jayne 2005. Inverse PCR. In: Fuchs, J. and Podda, M. eds. Encyclopedia of Medical Genomics and Proteomics, New York: Marcel Dekker, pp. 687-692. (10.3109/9780203997352.138)

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Polymerase chain reaction (PCR) is widely accepted as one of the most powerful tools in molecular biology capable of amplifying over 106 identical copies of small (few kb), specific DNA regions. It utilizes the thermostability of the enzyme Taq DNA polymerase I, and allows quick and efficient exponential amplification of a target region between known DNA sequences. This removes the need for time‐consuming cloning, although it does have two key limitations. It is only efficient in amplifying relatively small DNA fragments and secondly, but more importantly, PCR can only be used to amplify regions of known DNA sequence. As such, PCR alone cannot be used to amplify DNA sequences adjacent to an unknown sequence, thus making chromosomal walking unfeasible.

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: genomics, proteomics, gene therapy, viruses, DNA, FISH, microarrays, nucleotides, bacteria
Publisher: Marcel Dekker
ISBN: 9780824755645
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Last Modified: 05 Jun 2017 03:28

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