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Hyaline cartilage tissue is formed through the co-culture of passaged human chondrocytes and primary bovine chondrocytes

Taylor, Drew W., Ahmed, Naziss, Hayes, Anthony Joseph, Ferguson, Peter, Gross, Allan E., Caterson, Bruce and Kandel, Rita A. 2012. Hyaline cartilage tissue is formed through the co-culture of passaged human chondrocytes and primary bovine chondrocytes. Journal of Histochemistry & Cytochemistry 60 (8) , pp. 576-587. 10.1369/0022155412449018

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Abstract

To circumvent the problem of sufficient number of cells for cartilage engineering, we previously developed a two stage culture system to redifferentiate monolayer culture-expanded dedifferentiated human articular chondrocytes by co-culture with primary bovine chondrocytes (bP0). The aim of this study was to analyze the composition of the cartilage tissue formed in stage one and compare it to bP0 grown alone to determine the optimal length of the co-culture stage of the system. Biochemical data shows that the extracellular matrix accumulation was evident after 2 weeks of co-culture which was one week behind the bP0 control culture. By 3-4 weeks the amounts of accumulated proteoglycans and collagens were comparable. Expression of chondrogenic genes, Sox 9, aggrecan and collagen type II, was also at similar levels by week 3 of culture. Immunohistochemical staining of both co-culture and control tissues showed accumulation of type II collagen, aggrecan, biglycan, decorin, and chondroitin sulphate in appropriate zonal distributions. These data indicate that co-cultured cells form cartilaginous tissue that starts to resemble that formed by bP0 after 3 weeks suggesting that the optimal time to terminate the co-culture stage, isolate the now redifferentiated cells and to start stage two is just after 3 weeks.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QP Physiology
R Medicine > RC Internal medicine
Uncontrolled Keywords: Tissue regeneration, articular cartilage, de-differentiation, chondrocytes,
Additional Information: Published online before print.
Publisher: SAGE Publications
ISSN: 0022-1554
Last Modified: 09 Feb 2019 23:10
URI: http://orca.cf.ac.uk/id/eprint/33624

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