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Determination of nitroglycerin and its dinitrate metabolites in human plasma by high-performance liquid chromatography with thermal energy analyzer detection

Woodward, Amanda J., Lewis, P. A., Aylward, Mansel, Rudman, R. and Maddock, J. 1984. Determination of nitroglycerin and its dinitrate metabolites in human plasma by high-performance liquid chromatography with thermal energy analyzer detection. Journal of Pharmaceutical Sciences 73 (12) , pp. 1838-1840. 10.1002/jps.2600731249

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Abstract

Highly selective and sensitive high-performance liquid chromatographic assay employing a thermal energy analyzer as the detector for nitroglycerin and its dinitrate metabolites in human plasma has been developed. Prior to chromatography the method employs a simple one-stage extraction step. Nitroglycerin and its dinitrate metabolites are then chromato-graphed on a 10-μm nitrile bonded phase column using an internal-external standard method. The nitroglycerin and its 1,2,3-propanetriol-1,3- and -1,2-dinitrate metabolites (glyceryl-1,3- and -1,2-dinitrate) have a retention time of 8.5, 10.5, and 11.5 min, respectively at a flow rate of 2.0 mL/min for a mobile phase of 5% v/v acetone in n-hexane. The limits of sensitivity were 0.05 ng/mL for nitroglycerin and 0.25 ng/mL for the dinitrate metabolites. Linearity of response was observed over the 0.1-2.0-ng/mL range for nitroglycerin and 0.5-10.0-ng/mL range for the dinitrate metabolites. Blood level data from a pilot study with human volunteers in receipt of an oral form of nitroglycerin is presented.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords: nitroglycerin—HPLC, parent drug, dinitrate metabolites, human plasma, thermal energy analyzer—HPLC, nitroglycerin, dinitrate metabolites, human plasma
Publisher: WileyBlackwell
ISSN: 0022-3549
Last Modified: 10 Nov 2019 01:37
URI: http://orca.cf.ac.uk/id/eprint/36534

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