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Use of a non-extraction HPLC technique for measuring angiotensin-converting enzyme under optimum conditions

Badminton, Michael Norman, Dawson, Christine M., Rainbow, Sandra J. and Tickner, T.R. 1991. Use of a non-extraction HPLC technique for measuring angiotensin-converting enzyme under optimum conditions. Annals of Clinical Biochemistry 28 (4) , pp. 396-400.

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Abstract

A non-extraction high performance liquid chromatography assisted method for the measurement of angiotensin-converting enzyme in serum has been developed. Serum containing the enzyme was incubated with the synthetic substrate, furylacryloylphenylalanylglycylglycine at a concentration approaching 10 times the apparent Km, prior to injection onto a shielded hydrophobic reverse phase high performance liquid chromatography column. This allowed the product to be separated and measured directly without the need for a time consuming protein extraction step. The method is enzymatically sound and avoids the methodological problems associated with automated kinetic assays.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Wiley-Blackwell
ISSN: 0004-5632
Related URLs:
Last Modified: 04 Jun 2017 04:20
URI: http://orca.cf.ac.uk/id/eprint/37285

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