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A perioculomotor nitridergic population in the macaque and cat

Erichsen, Jonathan Thor and May, Paul J. 2012. A perioculomotor nitridergic population in the macaque and cat. Investigative Ophthalmology and Visual Science 53 (9) , pp. 5751-5761. 10.1167/iovs.12-10287

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Purpose. To determine the distribution of cells containing synthetic enzymes for the unconventional neurotransmitter, nitric oxide, with respect to the known populations within the oculomotor complex. Methods. The oculomotor complex was investigated in monkeys and cats by use of histochemistry to demonstrate nicotinamide adenine dinucleotide phosphate diaphorase positive (NADPHd+) cells and antibodies to localize neuronal nitric oxide synthase positive (NOS+) cells. In some cases, wheatgerm agglutinin conjugated horseradish peroxidase (WGA-HRP) was injected into extraocular muscles to allow comparison of retrogradely labeled and NADPHd+ cell distributions. Results. The distribution of the NADPHd+ and NOS+ neurons did not coincide with that of preganglionic and extraocular motoneurons in the oculomotor complex. However, labeled perioculomotor neurons were observed. Specifically, in monkeys, they lay in an arc that extended from between the oculomotor nuclei into the supraoculomotor area (SOA). Comparison of WGA-HRP labeled medial and superior rectus motoneurons with NADPHd staining confirmed that the distributions overlapped, but showed that the C- and S-group cells were not NADPHd+. This suggested that NADPHd+ cells are part of the centrally projecting Edinger-Westphal population (EWcp). Examination of the NADPHd+ cell distribution in the cat showed that these cells were indeed found primarily within its well-defined EWcp. Conclusions. Based on their similar distributions, it appears that the peptidergic EWcp neurons, which project widely in the brain, may also be nitridergic. While the preganglionic and C- and S- group motoneuron populations do not utilize this non-synaptic neurotransmitter, nitric oxide produced by surrounding NADPHd+ cells may modulate the activity of these motoneurons.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
Additional Information: Confirmation received by publisher on 21 February 2014 that publisher's pdf can be self-archived 6 months after publication.
Publisher: Association for Research in Vision and Ophthalmology
ISSN: 0146-0404
Date of First Compliant Deposit: 30 March 2016
Last Modified: 04 Jun 2017 04:21

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