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A Consensus on Fungal Polymerase Chain Reaction Diagnosis?: A United Kingdom-Ireland Evaluation of Polymerase Chain Reaction Methods for Detection of Systemic Fungal Infections

White, P. Lewis, Barton, Richard, Guiver, Malcolm, Linton, Christopher J., Wilson, Steve, Smith, Melvyn, Gomez, Beatriz L., Carr, Michael J., Kimmitt, Patrick T., Seaton, Shila, Rajakumar, Kumar, Holyoake, Tessa, Kibbler, Chris C., Johnson, Elizabeth, Hobson, Richard P., Jones, Brian and Barnes, Rosemary Ann 2006. A Consensus on Fungal Polymerase Chain Reaction Diagnosis?: A United Kingdom-Ireland Evaluation of Polymerase Chain Reaction Methods for Detection of Systemic Fungal Infections. The Journal of Molecular Diagnostics 8 (3) , pp. 376-384. 10.2353/jmoldx.2006.050120

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Abstract

The limitations of classical diagnostic methods for invasive fungal infections (IFIs) have led to the development of molecular techniques to aid in the detection of IFIs. Despite good published performance, interlaboratory reproduction of these assays is variable, and no consensus has been reached for an optimal method. This publication describes the first multicenter study of polymerase chain reaction methods, for the detection of Aspergillus and Candida species, currently used in the UK and Ireland by distribution and analysis of multiple specimen control panels. All three Candida methods were comparable, achieving a satisfactory level of detection (10 cfu), and the method of preference was dependent on the requirements of the particular laboratory. The results for the five Aspergillus assays were more variable, but two methods (2Asp and 4Asp) were superior (101 conidia). Formally, the overall performances of the two Aspergillus assays were comparable (statistic 0.77). However, on the Roche LightCycler, there was a clear sample-type effect that greatly reduced the detection limit of the 4Asp method when testing whole blood samples. Therefore, the preferred Aspergillus method relied on the amplification platform available to the user. This study represents the initial process to achieve a consensus method for the diagnosis of IFIs.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: Q Science > QR Microbiology
R Medicine > R Medicine (General)
R Medicine > RM Therapeutics. Pharmacology
Publisher: Elsevier
ISSN: 1525-1578
Last Modified: 04 Jun 2017 04:25
URI: http://orca.cf.ac.uk/id/eprint/38729

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