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Residue choice defines efficiency and influence of bioorthogonal protein modification via genetically encoded strain promoted Click chemistry

Reddington, Samuel C., Tippmann, Eric Michael and Jones, Darran Dafydd 2012. Residue choice defines efficiency and influence of bioorthogonal protein modification via genetically encoded strain promoted Click chemistry. Chemical Communications 48 (67) , pp. 8419-8421. 10.1039/c2cc31887c

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Abstract

GFP and a FRET compatible dye were used to assess the influence of genetically encoded aryl azide positioning on Click chemistry-based protein conjugation. While modification efficiency of the sampled mutants using a strain promoted reaction varied by as much as [similar]10 fold, there was no simple correlation with accessibility of the aryl azide on GFP's surface. One labeled GFP mutant (Gln204AzPhe) exhibited high efficiency FRET ([similar]90%) and an unprecedented pseudo-Stokes shift of 126 nm.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Chemistry
Subjects: Q Science > QD Chemistry
Publisher: Royal Society of Chemistry
ISSN: 1359-7345
Last Modified: 10 Mar 2020 15:01
URI: http://orca.cf.ac.uk/id/eprint/38925

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