Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Inhaled particulate matter causes expression of nuclear factor (NF)-kappa B-related genes and oxidant-dependent NF-kappa B activation in vitro

Shukla, Arti, Timblin, Cynthia, Berube, Kelly Ann, Gordon, Terry, McKinney, Willie, Driscoll, Kevin, Vacek, Pamela and Mossman, Brooke T. 2000. Inhaled particulate matter causes expression of nuclear factor (NF)-kappa B-related genes and oxidant-dependent NF-kappa B activation in vitro. American Journal of Respiratory Cell and Molecular Biology 23 (2) , pp. 182-187.

Full text not available from this repository.

Abstract

High levels of ambient air pollution are associated with exacerbation of asthma and respiratory morbidity, yet little is known concerning the mechanisms of inflammation and toxicity by components of inhaled particulate matter (PM). Brief inhalation of PM2.5 (particles of an aerodynamic diameter of < 2.5 microns) (300 μ g/m3 air for 6 h followed by a period of 24 h in clean air) by either C3H/HeJ or C57/BL6 mice caused significant (P ⩽ 0.05) increases in steady-state messenger RNA (mRNA) levels of a number of nuclear factor (NF)- κ B–associated and/ or –regulated genes, including tumor necrosis factor- α and - β , interleukin-6, interferon- γ , and transforming growth factor- β . Lung mRNA levels of lymphotoxin- β and macrophage migration inhibitory factor were unchanged. In murine C10 alveolar cells and an NF- κ B–luciferase reporter cell line, exposure to PM2.5 at noncytotoxic concentrations resulted in increases in transcriptional activation of NF- κ B–dependent gene expression which were inhibited in the presence of catalase. Early and persistent increases in intracellular oxidants, as measured by flow cytometry and cell imaging using the oxidant probe 2 ′ -7 ′ -dichlorofluoroscin diacetate, were observed in epithelial cells exposed to PM2.5 and ultrafine carbon black particles. Studies here are the first to show NF- κ B–related inflammatory and cytokine gene expression after inhalation of PM2.5 and oxidant-dependent induction of NF- κ B activity by PM2.5 in pulmonary epithelial cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QR Microbiology
Publisher: American Thoracic Society
ISSN: 1044-1549
Last Modified: 04 Jun 2017 04:44
URI: http://orca.cf.ac.uk/id/eprint/43700

Actions (repository staff only)

Edit Item Edit Item