Abnormal metabolic fate of nitric oxide in Type I diabetes mellitus

Milsom, Alexandra Barrie, Jones, Christopher John Hugh, Goodfellow, Jonathan, Frenneaux, Michael Paul, Peters, J. R. and James, Philip Eurig 2002. Abnormal metabolic fate of nitric oxide in Type I diabetes mellitus. Diabetologia 45 (11) , pp. 1515-1522. 10.1007/s00125-002-0956-9

Full text not available from this repository.

Abstract

Aims/hypothesis. Reduced bioavailability of endothelium-derived nitric oxide is implicated in diabetic macrovascular and microvascular disease. In patients with diabetes, we hypothesised that protein glycosylation can alter nitric oxide binding affinity of haemoglobin and plasma proteins, hence reducing nitric oxide availability and causing an alteration in nitric oxide metabolism. Methods. Binding of nitric oxide to haemoglobin was studied across a range of glycosylation levels in vitro (HbA1c 5.9 to 9.8%). In clinical studies nitrate, nitrite, nitrosyl haemoglobin and plasma nitrosothiols were measured in venous blood from 23 patients with uncomplicated Type I (insulin-dependent) diabetes mellitus and 17 non-diabetic control subjects. Samples were analysed at baseline and after nitric oxide was added ex vivo. Results. Nitric oxide-haemoglobin binding was increased at a HbA1c greater than 8.5% compared with 5.9% (p<0.01). Basal nitrosyl haemoglobin was higher in diabetic patients compared with the control subjects (0.59-0.12

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	Haemoglobin; glycosylation; nitric oxide; diabetes mellitus; bioavailability; metabolism; nitrosyl haemoglobin.
ISSN:	1432-0428
Last Modified:	18 Oct 2017 08:10
URI:	https://orca.cardiff.ac.uk/id/eprint/44

Citation Data

Cited 63 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item