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Syndecan-4 up-regulation in proliferative renal disease is related to microfilament organization

Yung, Susan, Woods, Anne, Chan, Tak Mao, Davies, Malcolm, Williams, John David and Couchman, John R. 2001. Syndecan-4 up-regulation in proliferative renal disease is related to microfilament organization. The FASEB Journal 15 (9) , pp. 1631-1633. 10.1096/fj.00-0794fje

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Abstract

Syndecan-4 is a transmembrane heparan sulfate proteoglycan (HSPG) expressed widely in mammalian cells. It has been implicated in growth-factor binding, cell-extracellular matrix adhesion, and tissue damage responses. Although several HSPGs are present within kidney mesangium and glomerular basement membranes, no data are available on the synthesis of syndecan-4 by mesangial cells or its expression in human renal disease. We examined renal biopsy specimens from normal controls, nonproliferative disease (thin-membrane disease), and progressive proliferative disease (IgA nephropathy). By using RT-PCR and immunohistochemical staining, we identified an increase in both gene expression (IgA nephropathy vs. thin membrane disease, P = 0.0004) and synthesis of syndecan-4 in progressive proliferative disease. Syndecan-4 increased within both mesangium and tubulo-interstitium, as did a-actinin, a microfilament cytoskeletal component. Syndecan-4 was a focal adhesion component in human mesangial cells, colocalizing with vinculin and a-actinin, and was present in the cortical, submembraneous myosin sheath as seen for a-actinin. Both syndecan-4 and a-actinin were retained selectively in detergent-resistant cytoskeleton-matrix preparations, emphasizing their close association in cell-matrix adhesion. Syndecan-4 may be important in the adhesion, migration, and proliferation of HMC, and its up-regulation could indicate proliferative disease.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: proteoglycans; heparan sulfate; actin; a-actinin
ISSN: 1530-6860
Last Modified: 30 Jun 2017 01:31
URI: http://orca.cf.ac.uk/id/eprint/454

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