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The effect of alginate oligosaccharides on the mechanical properties of Gram-negative biofilms

Powell, Lydia C., Sowedan, Ahmed, Khan, Saira, Wright, Chris J., Hawkins, Karl, Onsøyen, Edvar, Myrvold, Rolf, Hill, Katja E. and Thomas, David William 2013. The effect of alginate oligosaccharides on the mechanical properties of Gram-negative biofilms. Biofouling 29 (4) , pp. 413-421. 10.1080/08927014.2013.777954

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Abstract

The influence of a novel, safe antibiofilm therapy on the mechanical properties of Pseudomonas aeruginosa and Acinetobacter baumannii biofilms in vitro was characterized. A multiscale approach employing atomic force microscopy (AFM) and rheometry was used to quantify the mechanical disruption of the biofilms by a therapeutic polymer based on a low-molecular weight alginate oligosaccharide (OligoG). AFM demonstrated structural alterations in the biofilms exposed to OligoG, with significantly lower Young’s moduli than the untreated biofilms, (149 MPa vs 242 MPa; p < 0.05), a decreased resistance to hydrodynamic shear and an increased surface irregularity (Ra) in the untreated controls (35.2 nm ± 7.6 vs 12.1 nm ± 5.4; p < 0.05). Rheology demonstrated that increasing clinically relevant concentrations of OligoG (<10%) were associated with an increasing phase angle (δ) over a wide range of frequencies (0.1–10 Hz). These results highlight the utility of these techniques for the study of three-dimensional biofilms and for quantifying novel disruption therapies in vitro.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Systems Immunity Research Institute (SIURI)
Subjects: R Medicine > RK Dentistry
Uncontrolled Keywords: atomic force microscopy, rheology, biofilms, Pseudomonas aeruginosa
Publisher: Taylor & Francis
ISSN: 0892-7014
Last Modified: 04 Jun 2017 04:54
URI: http://orca.cf.ac.uk/id/eprint/46337

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