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Cell cycle-dependent Ca2+ oscillations in mouse embryos are regulated by nuclear targeting of PLCzeta

Larman, Mark Graham, Saunders, Christopher Matthew, Carroll, John, Lai, Francis Anthony and Swann, Karl 2004. Cell cycle-dependent Ca2+ oscillations in mouse embryos are regulated by nuclear targeting of PLCzeta. Journal of cell science 117 (12) , pp. 2513-2521. 10.1242/jcs.01109

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During the first cell cycle Ca2+ oscillations are regulated in a cell cycle-dependent manner, such that the oscillations are unique to M phase. How the Ca2+ oscillations are regulated with such cell cycle stage-dependency is unknown, despite their importance for egg activation and embryo development. We recently identified a novel, sperm-specific phospholipase C (PLCzeta; PLC) that triggers Ca2+ oscillations similar to those caused by sperm. We show that PLC-induced Ca2+ oscillations also occur exclusively during M phase. The cell cycle-dependency can be explained by PLC's localisation to the pronuclei, which depends specifically upon a nuclear localisation signal sequence. Preventing pronuclear localisation of PLC by mutation of the nuclear localisation signal, or by inhibiting pronuclear formation/import, can prolong Ca2+ oscillations or allow them to occur during interphase. These data suggest a novel mechanism for regulating a PLC through nuclear sequestration and may explain the cell cycle-dependent regulation of Ca2+ oscillations following fertilisation.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Uncontrolled Keywords: Fertilisation ; Phospholipase C ; Ca2+ oscillations ; Cell cycle
Publisher: Company of Biologists
ISSN: 1477-9137
Last Modified: 27 Mar 2020 02:38

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