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Different photochemical events of a genetically encoded phenyl azide define and modulate GFP fluorescence

Reddington, Samuel C., Rizkallah, Pierre, Watson, Peter Duncan, Pearson, Rachel, Tippmann, Eric Michael and Jones, Darran Dafydd 2013. Different photochemical events of a genetically encoded phenyl azide define and modulate GFP fluorescence. Angewandte Chemie - International Edition 52 (23) , pp. 5974-5977. 10.1002/anie.201301490

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Abstract

Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Chemistry
Medicine
Subjects: Q Science > QD Chemistry
Uncontrolled Keywords: expanded genetic code; green fluorescent protein; phenyl azide; photochemistry; protein engineering
Publisher: Wiley-Blackwell
ISSN: 1433-7851
Funders: BBSRC, SARTRE, EPSRC, MRC
Last Modified: 14 Jul 2019 00:08
URI: http://orca.cf.ac.uk/id/eprint/48715

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