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A wide-angle X-ray fibre diffraction method for quantifying collagen orientation across large tissue areas: application to the human eyeball coat

Pijanka, Jacek K., Abass, Ahmed, Sorensen, Thomas, Elsheikh, Ahmed and Boote, Craig 2013. A wide-angle X-ray fibre diffraction method for quantifying collagen orientation across large tissue areas: application to the human eyeball coat. Journal of Applied Crystallography 46 (5) , pp. 1481-1489. 10.1107/S0021889813022358

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Abstract

A quantitative map of collagen fibril orientation across the human eyeball coat, including both the cornea and the sclera, has been obtained using a combination of synchrotron wide-angle X-ray scattering (WAXS) and three-dimensional point mapping. A macromolecular crystallography beamline, in a custom-modified fibre diffraction setup, was used to record the 1.6 nm intermolecular equatorial reflection from fibrillar collagen at 0.5 mm spatial resolution across a flat-mounted human eyeball coat. Fibril orientation, derived as an average measure of the tissue thickness, was quantified by extraction of the azimuthal distribution of WAXS scatter intensity. Vector plots of preferential fibre orientation were remapped onto an idealized eyeball surface using a custom-built numerical algorithm, to obtain a three-dimensional representation of the collagen fibril architecture.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
Uncontrolled Keywords: human eye; cornea; sclera; collagen; wide-angle X-ray fibre diffraction.
Publisher: International Union of Crystallography
ISSN: 0021-8898
Last Modified: 15 Nov 2017 22:57
URI: http://orca.cf.ac.uk/id/eprint/51109

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