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Interaction with surrounding normal epithelial cells influences signalling pathways and behaviour of Src-transformed cells

Kajita, Mihoko, Hogan, Catherine, Harris, Andrew R., Dupre-Crochet, Sophie, Itasaki, Nobue, Kawakami, Koichi, Charras, Guillaume, Tada, Masazumi and Fujita, Yasuyuki 2010. Interaction with surrounding normal epithelial cells influences signalling pathways and behaviour of Src-transformed cells. Journal of Cell Science 123 (2) , pp. 171-180. 10.1242/jcs.057976

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Abstract

At the initial stage of carcinogenesis, transformation occurs in a single cell within an epithelial sheet. However, it remains unknown what happens at the boundary between normal and transformed cells. Using Madin-Darby canine kidney (MDCK) cells transformed with temperature-sensitive v-Src, we have examined the interface between normal and Src-transformed epithelial cells. We show that Src-transformed cells are apically extruded when surrounded by normal cells, but not when Src cells alone are cultured, suggesting that apical extrusion occurs in a cell-context-dependent manner. We also observe apical extrusion of Src-transformed cells in the enveloping layer of zebrafish gastrula embryos. When Src-transformed MDCK cells are surrounded by normal MDCK cells, myosin-II and focal adhesion kinase (FAK) are activated in Src cells, which further activate downstream mitogen-activated protein kinase (MAPK). Importantly, activation of these signalling pathways depends on the presence of surrounding normal cells and plays a crucial role in apical extrusion of Src cells. Collectively, these results indicate that interaction with surrounding normal epithelial cells influences the signalling pathways and behaviour of Src-transformed cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
European Cancer Stem Cell Research Institute (ECSCRI)
Subjects: Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords: Apical extrusion; Focal adhesion kinase; Mitogen-activated protein kinase; Myosin-II; Src; Zebrafish
Publisher: The Company of Biologists
ISSN: 0021-9533
Last Modified: 04 Jun 2017 05:21
URI: http://orca.cf.ac.uk/id/eprint/51130

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