New methods for preparing, imaging and typifying desmids (Chlorophyta, Zygnematophyceae), including extended depth of focus and 3-D reconstruction

Mann, David G., Bayer, Misha M., Droop, Stephen J. M., Hicks, Yulia Alexandrovna ORCID: https://orcid.org/0000-0002-7179-4587, Marshall, Andrew David ORCID: https://orcid.org/0000-0003-2789-1395, Martin, Ralph Robert and Rosin, Paul L. ORCID: https://orcid.org/0000-0002-4965-3884 2007. New methods for preparing, imaging and typifying desmids (Chlorophyta, Zygnematophyceae), including extended depth of focus and 3-D reconstruction. Phycologia 46 (1) , pp. 29-45. 10.2216/04-60.1

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Abstract

Species- and genus-level taxonomy of desmids depends largely on shape and detail of the cell wall and chloroplast morphology. The depth of most desmid semicells, relative to the focal depth of conventional light microscopes, means that morphological characteristics are usually illustrated by drawings, made from material that is mounted in water to allow reorientation of specimens to different aspects of shape and pattern. Though a productive approach for two centuries, this has the disadvantages that features not initially detected or thought irrelevant are not recorded, drawing quality is variable, and individual specimens are rarely retained for further study. We describe methods for making permanent preparations of desmid cell walls and using these to produce extended depth of focus summary images and three-dimensional (3-D) reconstructions. Together with World-Wide Web dissemination of image stacks, these advances make it practical to make a desirable change from typification via drawings to typification via single or multiple preserved specimens. They will also facilitate standardization of taxon concepts and identification.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Computer Science & Informatics Engineering
Subjects:	Q Science > QA Mathematics > QA75 Electronic computers. Computer science
Uncontrolled Keywords:	Chlorophyta, Desmids, Extended depth of focus, Imaging, Microscopy, Taxonomy, Typification, World-Wide Web, Zygnematophyceae
Publisher:	Blackwell Publishing
ISSN:	0031-8884
Last Modified:	17 Oct 2022 09:41
URI:	https://orca.cardiff.ac.uk/id/eprint/5218

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