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Action of caffeine upon calcium accumulation by sarcoplasmic reticulum preparations isolated from rabbit heart [Abstract]

Blayney, Lynda Mary, Henderson, A. H., Thomas, H. and Muir, J. R. 1977. Action of caffeine upon calcium accumulation by sarcoplasmic reticulum preparations isolated from rabbit heart [Abstract]. Heart 39 (3) , p. 350. 10.1136/hrt.39.3.340

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Abstract

Caffeine partly influences mechanical performance of heart muscle by affecting internal membrane function. In skeletal and heart muscle caffeine alters calcium ac- cumulation by isolated sarcoplasmic reticulum fractions but the manner of its action is uncertain. Sarcoplasmic reticulum fractions were isolated from rabbit heart by differential centrifugation. Calcium accumulation, with and without oxalate, was followed using dual-wavelength spectrophotometry with murexide at 35°C. The calcium accumulation data were analysed according to first order kinetics. ATPase activity (basic and calcium activated) was also measured. Calcium accumulation both with and without oxalate was inhibited in a dose related manner by caffeine. At low but not high concentrations calcium activated ATPase activity was increased by caffeine but in the presence of oxalate it was not altered by caffeine at any calcium concentration. Caffeine did not alter the basic ATPase activity. The findings support the con- clusion that caffeine reduced calcium accumulation by altering the passive permeability of the sarcoplasmic recticulum vesicles to calcium.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Additional Information: From section in Journal containing Proceedings of the British Cardiac Society Autumn Meeting, 4-5 November 1976, Imperial College London. Abstracts of papers.
Publisher: BMJ Publishing Group
ISSN: 1355-6037
Last Modified: 31 Jan 2020 04:19
URI: http://orca.cf.ac.uk/id/eprint/52215

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