Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Characterization of the interactions between protein and carbon black

Chen, Tzu-Tao, Chuang, Kai-Jen, Chiang, Ling-Ling, Chen, Chun-Chao, Yeh, Chi-Tai, Wang, Liang-Shun, Gregory, Clive James, Jones, Timothy Peter, Berube, Kelly Ann, Lee, Chun-Nin, Chuang, Hsiao-Chi and Cheng, Tsun-Jen 2014. Characterization of the interactions between protein and carbon black. Journal of Hazardous Materials 264 , pp. 127-135. 10.1016/j.jhazmat.2013.10.055

Full text not available from this repository.

Abstract

A considerable amount of studies have been conducted to investigate the interactions of biological fluids with nanoparticle surfaces, which exhibit a high affinity for proteins and particles. However, the mechanisms underlying these interactions have not been elucidated, particularly as they relate to human health. Using bovine serum albumin (BSA) and mice bronchoalveolar lavage fluid (BALF) as models for protein–particle conjugates, we characterized the physicochemical modifications of carbon blacks (CB) with 23 nm or 65 nm in diameter after protein treatment. Adsorbed BALF-containing proteins were quantified and identified by pathways, biological analyses and protein classification. Significant modifications of the physicochemistry of CB were induced by the addition of BSA. Enzyme modulators and hydrolase predominately interacted with CB, with protein-to-CB interactions that were associated with the coagulation pathways. Additionally, our results revealed that an acute-phase response could be activated by these proteins. With regard to human health, the present study revealed that the CB can react with proteins (∼55 kDa and 70 kDa) after inhalation and may modify the functional structures of lung proteins, leading to the activation of acute-inflammatory responses in the lungs.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Earth and Ocean Sciences
Medicine
Subjects: Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords: Coagulation; Inflammation; Nanoparticle; Proteomics
Publisher: Elsevier
ISSN: 0304-3894
Date of Acceptance: 25 October 2013
Last Modified: 14 Apr 2019 22:18
URI: http://orca.cf.ac.uk/id/eprint/53943

Citation Data

Cited 2 times in Google Scholar. View in Google Scholar

Cited 8 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item