Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Chemically-specific dual/differential CARS micro-spectroscopy of saturated and unsaturated lipid droplets

Di Napoli, Claudia, Masia, Francesco, Pope, Iestyn, Otto, Cees, Langbein, Wolfgang Werner and Borri, Paola 2014. Chemically-specific dual/differential CARS micro-spectroscopy of saturated and unsaturated lipid droplets. Journal of Biophotonics 7 (1-2) , pp. 68-76. 10.1002/jbio.201200197

[img]
Preview
PDF - Published Version
Available under License Creative Commons Attribution.

Download (670kB) | Preview

Abstract

We have investigated the ability of dual-frequency Coherent Antistokes Raman Scattering (D-CARS) micro-spectroscopy, based on femtosecond pulses (100 fs or 5 fs) spectrally focussed by glass dispersion, to distinguish the chemical composition of micron-sized lipid droplets consisting of different triglycerides types (poly-unsaturated glyceryl trilinolenate, mono-unsaturated glyceryl trioleate and saturated glyceryl tricaprylate and glyceryl tristearate) in a rapid and label-free way. A systematic comparison of Raman spectra with CARS and D-CARS spectra was used to identify D-CARS spectral signatures which distinguish the disordered poly-unsaturated lipids from the more ordered saturated ones both in the CH-stretch vibration region and in the fingerprint region, without the need for lengthy CARS multiplex acquisition and analysis. D-CARS images of the lipid droplets at few selected wavenumbers clearly resolved the lipid composition differences, and exemplify the potential of this technique for label-free chemically selective rapid imaging of cytosolic lipid droplets in living cells.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Physics and Astronomy
Biosciences
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: Multiphoton microscopy; nonlinear optics; Coherent Antistokes Raman Scattering; lipids
Publisher: Wiley-Blackwell
ISSN: 1864-063X
Funders: BBSRC, EPSRC
Date of First Compliant Deposit: 30 March 2016
Date of Acceptance: 6 November 2012
Last Modified: 15 May 2019 21:02
URI: http://orca.cf.ac.uk/id/eprint/56959

Citation Data

Cited 6 times in Google Scholar. View in Google Scholar

Cited 18 times in Scopus. View in Scopus. Powered By Scopus® Data

Cited 2 times in Web of Science. View in Web of Science.

Actions (repository staff only)

Edit Item Edit Item

Downloads

Downloads per month over past year

View more statistics