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Immunofluorescence staining of spindles, chromosomes, and kinetochores in human oocytes

Riris, Solon, Cawood, Suzanne, Gui, Liming, Serhal, Paul and Homer, Hayden A. 2013. Immunofluorescence staining of spindles, chromosomes, and kinetochores in human oocytes. In: Homer, Hayden A. ed. Mammalian Oocyte Regulation: Methods and Protocols, Methods in Molecular Biology, vol. 957. New York: Humana Press, pp. 179-187. (10.1007/978-1-62703-191-2_12)

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Understanding how human oocytes execute chromosome segregation is of paramount importance as errors in this process account for the overwhelming majority of human aneuploidies and increase exponentially with advancing female age. The spindle is the cellular apparatus responsible for separating chromosomes at anaphase. For accurate chromosome segregation, spindle microtubules must establish appropriately configured attachments to chromosomes via kinetochores. With regard to understanding the mechanistic basis for human aneuploidies therefore, it will be important to explore the molecular underpinnings of spindle structure and the interaction of its microtubules with chromosomes in human oocytes. Here we describe a technique for simultaneously immunolabelling chromosomes, spindle microtubules and kinetochores in human oocytes.

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Biosciences
European Cancer Stem Cell Research Institute (ECSCRI)
Subjects: Q Science > QP Physiology
Uncontrolled Keywords: Mammalian oocyte; Meiosis; Spindle; Kinetochore; Immunostaining; Confocal microscopy; Chromosome alignment
Publisher: Humana Press
ISBN: 9781627031905
ISSN: 1064-3745
Last Modified: 28 Jun 2019 02:02

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