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Intracellular oxygen: similar results from two methods of measurement using Phosphorescent nanoparticles

Lloyd, David, Williams, Catrin Ffion, Vijayalakshmi, K., Kombrabail, M., White, Nicholas, Hayes, Anthony, Aon, Miguel A. and Krishnamoorthy, G. 2014. Intracellular oxygen: similar results from two methods of measurement using Phosphorescent nanoparticles. Journal of Innovative Optical Health Sciences 7 (2) , 1350041. 10.1142/S1793545813500417

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Abstract

The ability to resolve the spatio-temporal complexity of intracellular O2 distribution is the "Holy Grail" of cellular physiology. In an effort to obtain a minimally invasive approach to the mapping of intracellular O2 tensions, two methods of phosphorescent lifetime imaging microscopy were compared in the current study and gave similar results. These were two-photon confocal laser scanning microscopy with pinhole shifting, and picosecond time-resolved epi-phosphorescence microscopy using a single 0.5 μm focused spot. Both methods utilized Ru coordination complex embedded nanoparticles (45 nm diameter) as the phosphorescent probe, excited using pulsed outputs of a titanium–sapphire Tsunami lasers (710–1050 nm).

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
Uncontrolled Keywords: Time-resolved phosphorescence; intracellular O2; two-photon excitation; pinhole shifting
Publisher: World Scientific
ISSN: 1793-5458
Date of First Compliant Deposit: 18 February 2019
Date of Acceptance: 1 September 2013
Last Modified: 16 Mar 2019 22:28
URI: http://orca.cf.ac.uk/id/eprint/57070

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