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Two type II keratin genes are localized on human chromosome 12

Popescu, N. C., Bowden, Paul Edward and DiPaolo, J. A. 1989. Two type II keratin genes are localized on human chromosome 12. Human Genetics 82 (2) , pp. 109-112.

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Human genomic DNA containing two type II keratin genes, one coding for keratin 1 (K1, a 68-kD basic protein) and another closely linked type II gene 10-15kb upstream (K?, gene product unknown), was isolated on a single cosmid clone. EcoRI restriction fragments of the cosmid were subcloned into pGEM-3Z, and specific probes comprising the Cterminal coding and 3'noncoding regions of the two genes were constructed. The type II keratin genes were localized by in situ hybridization of the subcloned probes to normal human lymphocyte chromosomes. In a total of 70 chromosome spreads hybridized with the K? probe (gHK?-3', PstI, 800bp), 36 of the 105 grains observed were on chromosome 12, and 32 of these were clustered on the long arm near the centromere (12q11-13). In 100 labeled metaphases hybridized with the K1 probe (gHK1-3', BamHI-PstI, 2100 bp), 53 grains localized to chromosome 12 and 46 of these were found in the same region (q11-13). Therefore, both the gene for human keratin 1, a specific marker for terminal differentiation in mammalian epidermis, and another closely linked unknown type II keratin gene (K?, 10-15kb upstream of K1) are on the long arm (q11-13) of human chromosome 12.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: Q Science > QH Natural history > QH426 Genetics
Publisher: Springer Verlag
ISSN: 0340-6717
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Last Modified: 12 Jun 2019 02:23

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