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Specific analysis of the intron 22 XbaI polymorphism of the human factor VIII gene using long-distance PCR

De Brasi, C. D., Bowen, Derrick John, Collins, Peter William and Larripa, I. B. 1999. Specific analysis of the intron 22 XbaI polymorphism of the human factor VIII gene using long-distance PCR. British Journal of Haematology 107 (3) , pp. 566-568. 10.1046/j.1365-2141.1999.01731.x

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Abstract

A rapid, non-radioactive, PCR-based method to genotype the XbaI restriction fragment length polymorphism of the human factor VIII gene is described. The method uses long-distance PCR followed by XbaI restriction digestion and agarose gel electrophoresis. The 6.6 kb amplification product includes a constant XbaI site, which provides a digestion control. The specificity of the method was challenged by a blind experiment with 16 genomic DNA samples previously genotyped by Southern blot analysis: a perfect correlation was obtained between genotypes determined using Southern blot and PCR.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: Genetics; Haemophilia A; Factor VIII gene; XbaI restriction fragment length polymorphism; Long-distance PCR.
Publisher: Wiley-Blackwell
ISSN: 0007-1048
Last Modified: 04 Jun 2017 06:12
URI: http://orca.cf.ac.uk/id/eprint/57305

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