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Photoaffinity labelling of the TSH receptor on FRTL5, cells

Furmaniak, Jadwiga, Hashim, Faisal A., Buckland, Paul Robert, Petersen, Vaughan B., Beever, Karen, Howells, Roger David and Rees Smith, Bernard 1987. Photoaffinity labelling of the TSH receptor on FRTL5, cells. FEBS Letters 215 (2) , pp. 316-322. 10.1016/0014-5793(87)80169-2

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Abstract

An investigation of the properties of TSH receptors on FRTL5 cells using affinity labelling with a 125 I-labelled photoactive derivative of TSH is described. Our studies suggest that FRTL5 cells contain 2 principal types of cell surface TSH receptors. One form, probably a precursor, consists of a single polypeptide chain (Mr 120 000) with an intrachain loop of amino acids formed by a disulphide bridge. The other type of receptor consists of a water-soluble A chain (Mr 55000) linked to an amphiphilic B chain (Mr 35000) by a disulphide bridge. The 2 chain structure is probably derived from the single chain 120 000 protein by enzymatic cleavage of peptide sequences within the loop of amino acids formed by the intrachain disulphide bridge.

Item Type: Article
Date Type: Publication
Status: Published
Schools: MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Medicine
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: Thyroid; Thyrotropin; TSH receptor; (FRTL5 cell)
Publisher: Elsevier
ISSN: 0014-5793
Last Modified: 05 Feb 2020 03:36
URI: http://orca.cf.ac.uk/id/eprint/57469

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