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A single, multiplex analysis for all relevant activating NRAS gene mutations using heteroduplex generators

Belli, Carolina, Bowen, Derrick John, De Brasi, Carlos and Larripa, Irene 2004. A single, multiplex analysis for all relevant activating NRAS gene mutations using heteroduplex generators. British Journal of Haematology 126 (4) , pp. 602-605. 10.1111/j.1365-2141.2004.05079.x

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Abstract

We describe a multiplex polymerase chain reaction (PCR)-based test that detected all relevant NRAS activating mutations using a single PCR followed directly by electrophoresis. The test uses a Universal Heteroduplex Generator (UHG) to detect exon-2 (codon 61) NRAS mutations in multiplex with an UHG for exon-1 (codons 12 and 13). The method differentiated all 19 relevant mutations in these exons and showed a mutation independent sensitivity of approximately 6%. The sensitive, specific detection of all NRAS activating mutations using this single rapid test represents a minimum workload and could be applied readily for large-scale screening and for routine analysis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: Universal Heteroduplex Generator; NRAS oncogene; multiplex assay
Publisher: Wiley-Blackwell
ISSN: 0007-1048
Last Modified: 04 Jun 2017 06:14
URI: http://orca.cf.ac.uk/id/eprint/57556

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