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Isolation and characterization of the gene encoding the a-subunit of the rat pituitary glycoprotein hormones

Burnside, Joan, Buckland, Paul Robert and Chin, William W. 1988. Isolation and characterization of the gene encoding the a-subunit of the rat pituitary glycoprotein hormones. Gene 70 (1) , pp. 67-74. 10.1016/0378-1119(88)90105-9

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The gene encoding the common a subunit of the rat pituitary glycoprotein hormones was isolated from a rat genomic DNA library. The gene spans approximately 8 kb, and contains four exons and three intervening sequences of 5.4 kb, 1.1 kb and 0.6 kb. Blot hybridization of restriction enzyme digests of rat genomic DNA suggests that the a gene is present in a single copy. The coding region and 424 bp of the 5' -flanking region of the gene were sequenced. Primer extension and S l nuclease analyses revealed a single transcriptional start point downstream from consensus promoter elements. The organization of the rat a-subunit gene is similar to that of the human and bovine genes including the sizes and locations of the four exons and three introns. In addition, a region of strong sequence similarity has been identified in the 5' -flanking region of the rat, human and bovine genes. This region includes sequences which are similar to a putative triiodothyronine regulatory element and the previously identified cAMP regulatory region; such sequences may mediate the known effects of these factors on a-subunit gene expression.

Item Type: Article
Date Type: Publication
Status: Published
Schools: MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: Recombinant DNA; exon; intron; genomic library; promoter; start point of transcription
Publisher: Elsevier
ISSN: 0378-1119
Last Modified: 05 Feb 2020 03:37

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