| Burnside, Joan, Buckland, Paul Robert and Chin, William W. 1988. Isolation and characterization of the gene encoding the a-subunit of the rat pituitary glycoprotein hormones. Gene 70 (1) , pp. 67-74. 10.1016/0378-1119(88)90105-9 |
Abstract
The gene encoding the common a subunit of the rat pituitary glycoprotein hormones was isolated from a rat genomic DNA library. The gene spans approximately 8 kb, and contains four exons and three intervening sequences of 5.4 kb, 1.1 kb and 0.6 kb. Blot hybridization of restriction enzyme digests of rat genomic DNA suggests that the a gene is present in a single copy. The coding region and 424 bp of the 5' -flanking region of the gene were sequenced. Primer extension and S l nuclease analyses revealed a single transcriptional start point downstream from consensus promoter elements. The organization of the rat a-subunit gene is similar to that of the human and bovine genes including the sizes and locations of the four exons and three introns. In addition, a region of strong sequence similarity has been identified in the 5' -flanking region of the rat, human and bovine genes. This region includes sequences which are similar to a putative triiodothyronine regulatory element and the previously identified cAMP regulatory region; such sequences may mediate the known effects of these factors on a-subunit gene expression.
| Item Type: | Article |
|---|---|
| Date Type: | Publication |
| Status: | Published |
| Schools: | MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG) Medicine |
| Subjects: | R Medicine > R Medicine (General) |
| Uncontrolled Keywords: | Recombinant DNA; exon; intron; genomic library; promoter; start point of transcription |
| Publisher: | Elsevier |
| ISSN: | 0378-1119 |
| Last Modified: | 05 Feb 2020 03:37 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/57616 |
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