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Reanalysis of P2X(7) receptor expression in rodent brain

Sim, J. A., Young, Mark Thomas, Sung, H. Y., North, R. A. and Surprenant, A. 2004. Reanalysis of P2X(7) receptor expression in rodent brain. Journal of Neuroscience 24 (28) , pp. 6307-6314. 10.1523/JNEUROSCI.1469-04.2004

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Abstract

P2X receptors are cationic-selective ion channels gated by extracellular ATP. There are seven subunits (P2X1-7), the first six of which are expressed throughout the peripheral and central nervous systems. P2X7 receptors are rapidly upregulated and activated as a result of inflammatory stimuli in immune cells, where they act not only as cationic channels but uniquely couple with rapid release of proinflammatory cytokines, cytoskeletal rearrangements, and apoptosis or necrotic cell death. The P2X7 receptor has been termed the cytolytic non-neuronal P2X receptor because it had not been detected in neurons until recently when it has been immunolocalized to several brain regions, particularly the hippocampus, and has been suggested to be involved in presynaptic modulation of transmitter release. Because its expression in brain neurons may have substantial functional implications, we have performed detailed immunocytochemical, immunoblot, and immunoprecipitation studies on brain and non-neuronal tissue using all currently available antibodies. We first examined rats, but staining patterns were inconsistent among antibodies; we therefore studied mice for which there are two P2X7 knock-out mice constructs available, one expressing the LacZ transgene. We found that P2X7 receptor protein is strongly and reliably detected in the submandibular gland and lung of wild-type mice but not in either of the P2X7-/- mice. However, we failed to find evidence for P2X7 receptor protein in hippocampal neurons or their input-output projections. Either the P2X7 protein in the hippocampus is below the limits of detection by the currently available methods or it is not present.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords: purinergic receptors; hippocampal neurons; immunocytochemistry; ligand-gated ion channels; transgenic animals; presynaptic inhibition
Additional Information: Pdf uploaded in accordance with publishers policy as at http://www.jneurosci.org/site/misc/ifa_policies.xhtml (accessed 03/03/2014)
Publisher: Society for Neuroscience
ISSN: 0270-6474
Date of First Compliant Deposit: 30 March 2016
Last Modified: 02 May 2019 12:13
URI: http://orca.cf.ac.uk/id/eprint/57843

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