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A molecular analysis of the telomeric end of the major histocompatibility complex: DNA typing of HLA-A3 subtypes and -B7

Dorak, M. Tevfik, Wilson, David W. L., Galbraith, Ian, Henderson, Nancy, Burnett, Alan Kenneth and Worwood, Mark 1995. A molecular analysis of the telomeric end of the major histocompatibility complex: DNA typing of HLA-A3 subtypes and -B7. Human Immunology 42 (1) , pp. 1-8. 10.1016/0198-8859(94)00054-T

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Abstract

A molecular investigation of the HLA-A, -B, -C, 182, D6S128, and D6S265 loci was carried out using RFLP and PCR analyses in 331 healthy individuals, 21 HLA-A3B7 homozygous subjects, and 17 HLA homozygous cell lines. New polymorphic RFLP patterns were noted at the HLA-A and -B loci which were useful for distinguishing subtypes of HLA-A3 and -B44. Strictly specific bands were observed for HLA-A3, -All, -B7, -B57, and -Cw4. Molecular identification of two HLA-A3 subtypes was possible by HLA-A RFLP and D6S265 PCR analyses. The two alleles of the 182 locus were associated with different HLA-A3 subtypes. It was shown that the serologically HLA-A3 homozygous cell line EHM was heterozygous for the two subtypes. The common subtype formed 91% of HLA-A3 antigens. Some serologically HLA-A3 homozygous healthy individuals were heterozygous for the subtypes. Using these new polymorphisms, a thorough molecular analysis of the haplotype HLA-A3B7DR15 at the three regions of the MHC was performed. The results have implications on HLA matching in transplantation, population genetics of the MHC, and disease association studies.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Elsevier
ISSN: 0198-8859
Last Modified: 18 Jan 2018 19:19
URI: http://orca.cf.ac.uk/id/eprint/58021

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