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Overgrowth of a leukemic culture by a minor CD34+ population

Baines, Paul, Lake, Helen, Fisher, Janet, Truran, Louise, Hoy, Terry and Burnett, Alan Kenneth 1998. Overgrowth of a leukemic culture by a minor CD34+ population. Leukemia Research 22 (6) , pp. 549-556. 10.1016/S0145-2126(98)00043-5

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We have investigated the differentiation potential of blast cells in a case of acute myeloid leukemia which comprised a majority CD34− population and a minor (2%) CD34+ fraction. Blasts were cultured for 2 weeks in a combination of cytokines—c-Kit ligand, interleukin 3 and granulocyte–macrophage colony-stimulating factor (SIGm mix)—together with all-trans retinoic acid or 1α,25-dihydroxy vitamin D3. Maturation of blasts was assessed by morphology on Romanowsky-stained slides, changes in surface CD markers and clonogenic culture. After 7 days of culture of unseparated blasts in SIGm, most maturation was monocytic, but with retinoic acid 63% of blasts had matured into granulocytes. Vitamin D3 enhanced monocytic differentiation, with 60% of cells becoming monocytic. The percentage of CD14 and CD15 positive cells decreased over 7 days in SIGm (from 62% to 17% and from 76% to 39% for CD14 and CD15, respectively). CD14+ cell numbers were maintained, or recovered, in cultures supplemented with vitamin D3 (59% at day 7), and CD15+ cell numbers, too, remained unchanged in the presence of retinoic acid (67%) or vitamin D3 (66%). Aberrant markers CD7 and CD56 declined under any conditions. When separated, both the CD34− and CD34+ fractions showed similar changes in morphology and surface maturation markers, suggesting that these two populations may be closely related. However, only a few CD34+ cells expressed the aberrant markers present on the majority blast population. The CD34− population declined in culture while the CD34+ fraction rapidly expanded. This probably reflects the difference in progenitor content; high numbers of colony-forming cells were concentrated in the CD34+ subpopulation. We conclude that both CD34− and CD34+ populations can differentiate but only the CD34+ fraction proliferates. Primitive clonogenic CD34+ cells from this patient may generate occasional aberrant CD34+ blasts which could then differentiate into the accumulating aberrant CD34− blast population.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Publisher: Elsevier
ISSN: 0145-2126
Last Modified: 18 Jan 2018 19:28

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