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Serum-free culture of human megakaryocyte progenitors

Masters, Gillian S. and Burnett, Alan Kenneth 1997. Serum-free culture of human megakaryocyte progenitors. Clinical and Laboratory Haematology 19 (1) , pp. 61-65. 10.1046/j.1365-2257.1997.00193.x

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Abstract

A serum-free method for the culture of megakaryocyte progenitor cells (burst-forming units megakaryocyte, BFU-Mk) from human bone marrow is described, using recombinant cytokines (interleukin(IL)-3, IL-6, IL-11 and stem cell factor [SCF]) to provide megakaryocyte colony-stimulating activity. IL-3 alone, but not IL-6, IL-11 or SCF alone, promoted BFU-Mk formation. The combination of IL-3 with IL-11 or with IL-6+SCF was necessary for optimal maturation of the megakaryocytes. No significant difference in BFU-Mk numbers was found between the combinations of IL-3+IL-11 and IL-3+IL-6+SCF. The development of BFU-Mk was found to be maximal at 12–14 d. Using these conditions, normal ranges for human bone marrow BFU-Mk were derived which may provide the basis for studying megakaryocyte colony growth in pathological conditions.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > RC Internal medicine
Uncontrolled Keywords: Megakaryocyte; progenitor; serum-free; IL-6; IL-11
Publisher: Wiley-Blackwell
ISSN: 0141-9854
Last Modified: 25 Jun 2017 04:42
URI: http://orca.cf.ac.uk/id/eprint/58946

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