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Identification of a retinoic acid responsive aldoketoreductase expressed in HL60 leukaemic cells

Mills, Kenneth Ian, Gilkes, Amanda, Sweeney, Marion, Choudhry, M. A., Woodgate, Louise, Bunce, C. M., Brown, G. and Burnett, Alan Kenneth 1998. Identification of a retinoic acid responsive aldoketoreductase expressed in HL60 leukaemic cells. FEBS Letters 440 (1-2) , pp. 158-162. 10.1016/s0014-5793(98)01435-5

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Abstract

Neutrophil and monocyte differentiation can be induced in HL60 leukaemia cells by all-trans-retinoic acid (ATRA) and 1alpha,25-dihydroxyvitamin D3 (D3), respectively, whose differentiating effects can be enhanced by exposure to 'anti-inflammatory agents' and steroids. We have provided evidence that this potentiation is via inhibition of the activity of an enzyme of the aldoketoreductase (AKR) family, but had failed to identify expression of known AKRs in HL60 cells. In this study, we have identified a previously unclassified aldoketoreductase family member (termed HAKR e) that is expressed in HL60 cells. HAKR e is dramatically and transiently up-regulated in HL60 cells within 24 h of exposure to ATRA, further supporting the proposition that a member(s) of this family of enzymes play(s) a role in controlling cell growth and/or differentiation.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
R Medicine > RZ Other systems of medicine
Uncontrolled Keywords: BlottingNorthern,BlottingWestern,Calcitriol/pharmacology,CellDifferentiation,CellDivision/drugeffects,GeneExpressionRegulationEnzymologic*,GeneLibrary,HL60Cells,Humans,HydroxysteroidDehydrogenases/antagonists&inhibitors,Indomethacin/pharmacology,Monocytes/cytology,Monocytes/enzymology*,Neutrophils/cytology,Neutrophils/enzymology*,Phagocytosis,RestrictionMapping,ReverseTranscriptasePolymeraseChainReaction,Tretinoin/pharmacology* Substances Tretinoin,Hydroxysteroid Dehydrogenases,Calcitriol,Indomethacin
Additional Information: Publication Types Research Support, Non-U.S. Gov't Full Text Sources Elsevier Science Gale Databases Other Literature Sources COS Scholar Universe Labome Researcher Resource - ExactAntigen/Labome Molecular Biology Databases TRANS-RETINOIC ACID - HSDB INDOMETHACIN - HSDB 1,25-DIHYDROXYCHOLECALCIFEROL - HSDB
Publisher: Elsevier
ISSN: 0014-5793
Last Modified: 20 Oct 2017 18:23
URI: http://orca.cf.ac.uk/id/eprint/59167

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