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Neutrophil elastase-mediated increase in airway temperature during inflammation

Schmidt, Annika, Belaaouaj, Azzaq, Bissinger, Rosi, Koller, Garrit, Malleret, Laurette, D'Orazio, Ciro, Facchinelli, Martino, Schulte-Hubbert, Bernhard, Molinaro, Antonio, Holst, Otto, Hammermann, Jutta, Schniederjans, Monika, Meyer, Keith C., Damkiaer, Soeren, Piacentini, Giorgio, Assael, Baroukh, Bruce, Kenneth, Häußler, Susanne, LiPuma, John J., Seelig, Joachim, Worlitzsch, Dieter and Döring, Gerd 2014. Neutrophil elastase-mediated increase in airway temperature during inflammation. Journal of Cystic Fibrosis 13 (6) , pp. 623-631. 10.1016/j.jcf.2014.03.004

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Abstract

Background How elevated temperature is generated during airway infections represents a hitherto unresolved physiological question. We hypothesized that innate immune defence mechanisms would increase luminal airway temperature during pulmonary infection. Methods We determined the temperature in the exhaled air of cystic fibrosis (CF) patients. To further test our hypothesis, a pouch inflammatory model using neutrophil elastase-deficient mice was employed. Next, the impact of temperature changes on the dominant CF pathogen Pseudomonas aeruginosa growth was tested by plating method and RNAseq. Results Here we show a temperature of ~38°C in neutrophil-dominated mucus plugs of chronically infected CF patients and implicate neutrophil elastase:α1-proteinase inhibitor complex formation as a relevant mechanism for the local temperature rise. Gene expression of the main pathogen in CF, P. aeruginosa, under anaerobic conditions at 38°C vs 30°C revealed increased virulence traits and characteristic cell wall changes. Conclusion Neutrophil elastase mediates increase in airway temperature, which may contribute to P. aeruginosa selection during the course of chronic infection in CF.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Postgraduate Medical and Dental Education
Subjects: R Medicine > RC Internal medicine
R Medicine > RM Therapeutics. Pharmacology
Uncontrolled Keywords: Neutrophil elastase, Temperature, Inflammation, Pseudomonas aeruginosa
Additional Information: Online publication date: 6 April 2014.
Publisher: Elsevier
ISSN: 1569-1993
Last Modified: 05 Mar 2019 11:01
URI: http://orca.cf.ac.uk/id/eprint/59210

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