Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Integrating vibrational signals, mitochondrial DNA and morphology for species determination in the genus Aphrodes(Hemiptera: Cicadellidae)

Bluemel, Joanna Katherine, Derlink, Maja, Pavlovcic, Petra, Russo, Isa-Rita, King, Robert Andrew, Corbett, Emma, Sherrard-Smith, Eleanor, Blejec, Andre, Wilson, Michael R., Stewart, Alan J. A., Symondson, William Oliver Christian and Virant-Doberlet, Meta 2014. Integrating vibrational signals, mitochondrial DNA and morphology for species determination in the genus Aphrodes(Hemiptera: Cicadellidae). Systematic Entomology 39 (2) , pp. 304-324. 10.1111/syen.12056

Full text not available from this repository.

Abstract

Reliable delimitation and identification of species is central not only to systematics, but also to studies of biodiversity, ecology and pest management. In the era of Internet‐based biodiversity databases misidentifications are rapidly disseminated and may have far‐reaching consequences. Leafhoppers from the genus Aphrodes (Hemiptera, Cicadellidae) are common and abundant, but, nevertheless, they are still a taxonomically challenging group whose members are often assessed in ecological studies and are also potential vectors of plant diseases. Previous study has shown that the syntype series for A. aestuarina (Edwards) includes also specimens of A. makarovi Zachvatkin and has suggested that misidentifications may be widespread in museum collections. We studied Aphrodes individuals collected from the U.K. and Slovenia in order to provide a more comprehensive analysis of this genus using multiple criteria. Combined work using male and female vibrational signals emitted during courtship, and a 600‐bp fragment within the barcoding region of the COI mtDNA gene, provided validated specimens that we also used for morphometric study. Analyses confirmed A. aestuarina, A. bicincta, A. diminuta and A. makarovi as behaviourally, genetically and morphologically distinct species. Although any of these approaches could be used alone to distinguish between species, combining morphological and molecular approaches will help to improve reliability, especially when identifying females. Morphological investigation of validated individuals from the U.K. and Slovenia also revealed geographic differences within species. By combining several body and aedeagus morphological characters males can be reliably identified, however, morphological differences between species are, nevertheless, relatively small. By contrast, observed genetic distances between Aphrodes species are relatively large (4.2–7.0%). At about half of our collecting sites more than one Aphrodes species was found and A. makarovi was collected together with every other species, including A. aestuarina on tidal saltmarshes. Due to low morphological variation between syntopic congeners it is likely that many museum specimens of Aphrodes have been assigned to the wrong species and species identification in ecological and vector studies may also be questionable.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QL Zoology
Publisher: Wiley-Blackwell
ISSN: 0307-6970
Last Modified: 16 May 2019 22:57
URI: http://orca.cf.ac.uk/id/eprint/59396

Citation Data

Cited 3 times in Google Scholar. View in Google Scholar

Cited 14 times in Scopus. View in Scopus. Powered By Scopus® Data

Cited 4 times in Web of Science. View in Web of Science.

Actions (repository staff only)

Edit Item Edit Item