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Monitoring of intra-ER free Ca2+

Gerasimenko, Julia Vladimirovna, Petersen, Ole Holger and Gerasimenko, Oleg Vsevolodovich 2014. Monitoring of intra-ER free Ca2+. Wiley Interdisciplinary Reviews: Membrane Transport and Signaling 3 (3) , pp. 63-71. 10.1002/wmts.106

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Abstract

The importance of calcium signaling in cell health and disease is the major driving force in current research of intracellular calcium homeostasis. Ca2+ release from the endoplasmic reticulum (ER) and other calcium stores seems to be the crucial factor in the activation of many cellular functions. Significant changes in ER Ca2+ content and dynamics have been implicated in the activation of the ER stress response, abnormal autophagy, and cell death which leads to a variety of pathological conditions. For example, in acute pancreatitis, an inflammatory disease of the exocrine pancreas caused primarily by bile stones or alcohol, excessive intracellular calcium overload due to Ca2+ release from internal stores followed by store operated Ca2+ entry (SOCE) leads to the premature activation of digestive proenzymes within pancreatic acinar cells. Recent data show that SOCE channel blockers are capable of substantially reducing the intracellular Ca2+ overload and subsequent cell necrosis without major alteration of ER Ca2+ content. We also demonstrate here that indirect ER measurements can be misleading and only direct intra-ER Ca2+ monitoring offers reliable conclusions. In this respect, it is essential to summarize the methods available and provide examples of direct measurements of free Ca2+ concentration [Ca2+] in the ER lumen in pancreatic acinar cells. This article is aimed at highlighting the major techniques for monitoring ER Ca2+ with reference to their advantages, limitations, and views for future improvements.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Systems Immunity Research Institute (SIURI)
Subjects: Q Science > Q Science (General)
Publisher: John Wiley & Sons
ISSN: 2190-460X
Last Modified: 16 May 2019 22:59
URI: http://orca.cf.ac.uk/id/eprint/59465

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