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Combined systems approaches reveal highly plastic responses to antimicrobial peptide challenge in escherichia coli

Kozlowska, Justyna, Vermeer, Louic S., Rogers, Geraint B., Rehnnuma, Nabila, Amos, Sarah-Beth T. A., Koller, Garrit, McArthur, Michael, Bruce, Kenneth D. and Mason, A. James 2014. Combined systems approaches reveal highly plastic responses to antimicrobial peptide challenge in escherichia coli. PLoS Pathogens 10 (5) , e1004104. 10.1371/journal.ppat.1004104

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Obtaining an in-depth understanding of the arms races between peptides comprising the innate immune response and bacterial pathogens is of fundamental interest and will inform the development of new antibacterial therapeutics. We investigated whether a whole organism view of antimicrobial peptide (AMP) challenge on Escherichia coli would provide a suitably sophisticated bacterial perspective on AMP mechanism of action. Selecting structurally and physically related AMPs but with expected differences in bactericidal strategy, we monitored changes in bacterial metabolomes, morphological features and gene expression following AMP challenge at sub-lethal concentrations. For each technique, the vast majority of changes were specific to each AMP, with such a plastic response indicating E. coli is highly capable of discriminating between specific antibiotic challenges. Analysis of the ontological profiles generated from the transcriptomic analyses suggests this approach can accurately predict the antibacterial mode of action, providing a fresh, novel perspective for previous functional and biophysical studies.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Postgraduate Medical and Dental Education
Subjects: Q Science > QR Microbiology
R Medicine > RM Therapeutics. Pharmacology
Publisher: Public Library of Science
ISSN: 1553-7374
Date of First Compliant Deposit: 30 March 2016
Last Modified: 05 Mar 2019 11:03

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