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Complex assembly, crystallization and preliminary X-ray crystallographic studies of MHC H-2Kd complexed with an HBV-core nonapeptide

Zhou, Minghai, Xu, Yanhui, Lou, Zhiyong, Cole, David, Li, Xiaojuan, Liu, Yiwei, Tien, Po, Rao, Zihe and Gao, George F. 2004. Complex assembly, crystallization and preliminary X-ray crystallographic studies of MHC H-2Kd complexed with an HBV-core nonapeptide. Acta Crystallographica Section D: Biological Crystallography 60 (8) , pp. 1473-1475. 10.1107/S0907444904013587

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Abstract

In order to establish a system for structural studies of the murine class I major histocompatibility antigen complex (MHC) H-2Kd, a bacterial expression system and in vitro refolding preparation of the complex of H-2Kd with human β2m and the immunodominant peptide SYVNTNMGL from hepatitis B virus (HBV) core-protein residues 87–95 was employed. The complex (45 kDa) was crystallized; the crystals belong to space group P2221, with unit-cell parameters a = 89.082, b = 110.398, c = 47.015 Å, α = β = γ = 90°. The crystals contain one complex per asymmetric unit and diffract X-­rays to at least 2.06 Å resolution. The structure has been solved by molecular replacement and is the first crystal structure of a peptide–H-2Kd complex.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Systems Immunity Research Institute (SIURI)
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: murine class I major histocompatibility antigen complex; hepatitis B virus-core nonapeptide
Publisher: International Union of Crystallography
ISSN: 0907-4449
Last Modified: 04 Jun 2017 06:28
URI: http://orca.cf.ac.uk/id/eprint/60525

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