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Multiple displacement amplification for generating an unlimited source of DNA for genotyping in nonhuman primate species

Ronn, A. -C., Andres, O., Bruford, Michael William, Crouau-Roy, B., Doxiadis, G., Domingo-Roura, X., Roeder, A. D., Verschoor, E., Zischler, H. and Syvanen, A. -C. 2006. Multiple displacement amplification for generating an unlimited source of DNA for genotyping in nonhuman primate species. International Journal of Primatology 27 (4) , pp. 1145-1169. 10.1007/s10764-006-9067-7

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Abstract

We evaluated a whole genome amplification method—multiple displacement amplification (MDA)—as a means to conserve valuable nonhuman primate samples. We tested 148 samples from a variety of species and sample sources, including blood, tissue, cell-lines, plucked hair and noninvasively collected semen. To evaluate genotyping success and accuracy of MDA, we used routine genotyping methods, including short tandem repeat (STR) analysis, denaturing gradient gel electrophoresis (DGGE), Alu repeat analysis, direct sequencing, and nucleotide detection by tag-array minisequencing. We compared genotyping results from MDA products to genotypes generated from the original (non-MD amplified) DNA samples. All genotyping methods showed good results with the MDA products as a DNA template, and for some samples MDA improved genotyping success. We show that the MDA procedure has the potential to provide a long-lasting source of DNA for genetic studies, which would be highly valuable for the primate research field, in which genetic resources are limited and for other species in which similar sampling constraints apply.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Sustainable Places Research Institute (PLACES)
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: Alu-SINE; minisequencing; multiple displacement amplification; short tandem repeat; single nucleotide polymorphism.
Publisher: Springer Verlag
ISSN: 0164-0291
Last Modified: 04 Jun 2017 06:30
URI: http://orca.cf.ac.uk/id/eprint/61112

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