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Histone deacetylase inhibitors increase neuronal differentiation in adult forebrain precursor cells

Siebzehnrubl, Florian, Buslei, Rolf, Eyupoglu, Ilker Y., Seufert, Sebastian, Hahnen, Eric and Blumcke, Ingmar 2007. Histone deacetylase inhibitors increase neuronal differentiation in adult forebrain precursor cells. Experimental Brain Research 176 (4) , pp. 672-678. 10.1007/s00221-006-0831-x

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Chromatin modification plays a key role in fate decision of neural stem cells. Here, we explored the impact of epigenetic remodelling onto neuronal fate determination using specific inhibitors of histone deacetylases (iHDAC). Adult subventricular zone (SVZ) precursor cells were expanded as neurospheres and treated in vitro with second generation iHDAC MS-275, M344 and suberoylanilide hydroxamic acid (SAHA). All tested compounds revealed a significant increase of betaIII-tubulin positive neurons (ranging from 258 to 431%) in a concentration-dependent manner. The number of oligodendrocytes was decreased by almost 50%, accompanied by a reduction of Olig2 mRNA expression. In contrast, astrocyte quantity remained unaffected after iHDAC treatment. Both control and iHDAC treated cells expressed markers of mature GABAergic and dopaminergic neurons. Increased expression levels of NeuroD, Cyclin D2 and B-lymphocyte translocation gene 3 (Btg3) point to a shift towards neuronal fate determination targeted by HDAC inhibitors.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
European Cancer Stem Cell Research Institute (ECSCRI)
Publisher: Springer
ISSN: 0014-4819
Last Modified: 04 Jun 2017 06:31

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