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Unmethlated domains in vertebrate DNA

Cooper, David Neil, Taggart, Mary H. and Bird, Adrian P. 1983. Unmethlated domains in vertebrate DNA. Nucleic Acids Research 11 (3) , pp. 647-658. 10.1093/nar/11.3.647

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Abstract

We have detected a fraction that is rich in unmethylated HpaII and Hhal sites by end-abelling HpaII fragments of chicken DNA. The fraction is not obvious when DNA fragments are stained with ethidium bromide as it amounts to less than 2% of the genome. The average frequency of sites for HpaII is over thirteen times greater in the unmethylated fraction than in total DNA. Partial digests indicate that the unmethylated sites are clustered in the genome. Similar unmethylated fractions were detected in six other vertebrates in both somatic and germ line DNA.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
ISSN: 0305-1048
Last Modified: 04 Jun 2017 06:33
URI: http://orca.cf.ac.uk/id/eprint/62093

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