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Microfluidic chip-based synthesis of alginate microspheres for encapsulation of immortalized human cells

Workman, Victoria Louise, Dunnett, Stephen Bruce, Kille, Peter and Palmer, D. D. 2007. Microfluidic chip-based synthesis of alginate microspheres for encapsulation of immortalized human cells. Biomicrofluidics 1 (1) , 014105. 10.1063/1.2431860

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Abstract

Cellular transplantation is a promising technology with great clinical potential in regenerative medicine and disease management. However, effective control over patient immunological response is essential. The encapsulation of cells within hydrogel microspheres is an increasingly prevalent method for the protection of cellular grafts from immune rejection. Microfluidic “chip” reactors present elegant solutions to several capsule generation issues, including the requirement for intercapsule uniformity, high reproducibility, and sterile, good manufacturing practice compliance. This study presents a novel method for the on-chip production of stable, highly monodisperse alginate microspheres and demonstrates its utility in the encapsulation of an immortalized human-derived cell line. Four populations of immortalized human embryonic kidney cells (HEK293) were encapsulated on chip within monodisperse alginate capsules. Cell viability measurements were recorded for each of the four encapsulated populations for 90days.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
ISSN: 1932-1058
Last Modified: 17 Mar 2019 23:58
URI: http://orca.cf.ac.uk/id/eprint/62344

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