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Rodent Aanat: intronic E-box sequences control tissue specificity but not rhythmic expression in the pineal gland

Humphries, Ann L., Wells, Timothy, Baler, Ruben, Klein, David C. and Carter, David Allan 2007. Rodent Aanat: intronic E-box sequences control tissue specificity but not rhythmic expression in the pineal gland. Molecular and Cellular Endocrinology 270 (1-2) , pp. 43-49. 10.1016/j.mce.2007.02.003

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Abstract

Arylalkylamine N-acetyltransferase (Aanat) is the penultimate enzyme in the serotonin–N-acetylserotonin–melatonin pathway. It is nearly exclusively expressed in the pineal gland and the retina. A marked rhythm of Aanat gene expression in the rat pineal is mediated by cyclic AMP response elements located in the promoter and first intron. Intron 1 also contains E-box elements, which mediate circadian gene expression in other cells. Here we examined whether these elements contribute to rhythmic Aanat expression in the pineal gland. This was done using transgenic rats carrying Aanat transgenes with mutant E-box elements. Circadian expression of Aanat transgenes was not altered by these mutations. However, these mutations enhanced ectopic expression establishing that the intronic Aanat E-box elements contribute to the gene's pineal specific expression. A similar role of the Aanat E-box has been reported in zebrafish, indicating that Aanat E-box mediated silencing is a conserved feature of vertebrate biology.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords: Arylalkylamine N-acetyltransferase; Pineal gland; Transgenic rat; E-box; Circadian rhythm
Publisher: Elsevier
ISSN: 0303-7207
Last Modified: 04 Jun 2017 06:35
URI: http://orca.cf.ac.uk/id/eprint/62418

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