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Metallothionein expression and Neutral Red uptake as biomarkers of metal exposure and effect in Eisenia fetida and Lumbricus terrestris exposed to Cd

Asensio, Vega, Kille, Peter, Morgan, Andrew John, Soto, Manu and Marigomez, Ionan 2007. Metallothionein expression and Neutral Red uptake as biomarkers of metal exposure and effect in Eisenia fetida and Lumbricus terrestris exposed to Cd. European Journal of Soil Biology 43 (Supp 1) , S233-S238. 10.1016/j.ejsobi.2007.08.022

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Abstract

In this study we compared short-term, sub-lethal responses of two different earthworm species, Eisenia fetida and Lumbricus terrestris, exposed to Cd (100 mg/kg) under laboratory conditions. Biological responses at the cellular and molecular genetic levels of organisation were measured. First, the Neutral Red uptake test was performed on extruded coelomocytes. Observations showed a significant reduction in cell membrane integrity in E. fetida exposed to Cd, whilst L. terrestris membranes appeared to be unaffected. Second, metallothionein 2 (MT2) gene expression levels were measured by Q-RT-PCR. Observations showed a significant up-regulation of the Cd-inducible metalloprotein in both species. Transcriptome measurements indicated that basal MT gene expression levels were higher in E. fetida than in L. terrestris, but Cd-induced MT2 up-regulation was approximately similar in samples from the two species. In conclusion, the susceptibility of coelomocyte membranes to Cd-evoked toxicosis is not correlated with Cd-induced MT2 expression levels.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: Neutral Red uptake; Metallothionein; Earthworms; Cadmium; Q-RT-PCR.
Publisher: Elsevier
ISSN: 1164-5563
Last Modified: 24 Jun 2017 10:42
URI: http://orca.cf.ac.uk/id/eprint/62646

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