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Differential global structural changes in the core particle of yeast and mouse proteasome induced by ligand binding

Arciniega, Marcelino, Beck, Phillipp, Lange, Oliver F., Groll, Michael and Huber, Robert 2014. Differential global structural changes in the core particle of yeast and mouse proteasome induced by ligand binding. Proceedings of the National Academy of Sciences of the United States of America 111 (26) , pp. 9479-9484. 10.1073/pnas.1408018111

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Abstract

Two clusters of configurations of the main proteolytic subunit β5 were identified by principal component analysis of crystal structures of the yeast proteasome core particle (yCP). The apo-cluster encompasses unliganded species and complexes with nonpeptidic ligands, and the pep-cluster comprises complexes with peptidic ligands. The murine constitutive CP structures conform to the yeast system, with the apo-form settled in the apo-cluster and the PR-957 (a peptidic ligand) complex in the pep-cluster. In striking contrast, the murine immune CP classifies into the pep-cluster in both the apo and the PR-957–liganded species. The two clusters differ essentially by multiple small structural changes and a domain motion enabling enclosure of the peptidic ligand and formation of specific hydrogen bonds in the pep-cluster. The immune CP species is in optimal peptide binding configuration also in its apo form. This favors productive ligand binding and may help to explain the generally increased functional activity of the immunoproteasome. Molecular dynamics simulations of the representative murine species are consistent with the experimentally observed configurations. A comparison of all 28 subunits of the unliganded species with the peptidic liganded forms demonstrates a greatly enhanced plasticity of β5 and suggests specific signaling pathways to other subunits.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Publisher: National Academy of Sciences
ISSN: 0027-8424
Last Modified: 19 Feb 2019 15:48
URI: http://orca.cf.ac.uk/id/eprint/62751

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