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Genetic engineering of neural function in transgenic rodents: towards a comprehensive strategy?

Wells, Timothy and Carter, David Allan 2001. Genetic engineering of neural function in transgenic rodents: towards a comprehensive strategy? Journal of Neuroscience Methods 108 (2) , pp. 111-130. 10.1016/S0165-0270(01)00391-0

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Abstract

As mammalian genome projects move towards completion, the attention of molecular neuroscientists is currently moving away from gene identification towards both cell-specific gene expression patterns (neuronal transcriptions) and protein expression/interactions (neuronal proteomics). In the long term, attention will increasingly be directed towards experimental interventions which are able to question neuronal function in a sophisticated manner that is cognisant of both transcriptomic and proteomic organization. Central to this effort will be the application of a new generation of transgenic approaches which are now evolving towards an appropriate level of molecular, temporal and spatial resolution. In this review, we summarize recent developments in transgenesis, and show how they have been applied in the principal model species for neuroscience, namely rats and mice. Current concepts of transgene design are also considered together with an overview of new genetically-encoded tools including both cellular indicators such as fluorescent activity reporters, and cellular regulators such as dominant negative signalling factors. Application of these tools in a whole animal context can be used to question both basic concepts of brain function, and also current concepts of underlying dysfuction in neurological diseases.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: Transgenesis; Transgene; Gene targeting; Gene promoter; Gene knockout; Rat; Mouse.
Publisher: Elsevier
ISSN: 0165-0270
Last Modified: 04 Jun 2017 06:38
URI: http://orca.cf.ac.uk/id/eprint/63052

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