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Utilization of amino acids in growing kidney proximal tubule cell cultures

Ali, Abdi Rashid and Evans, Peter J. 2001. Utilization of amino acids in growing kidney proximal tubule cell cultures. Cell Biology International 25 (5) , pp. 451-465. 10.1006/cbir.2000.0667

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The growth of rat kidney proximal tubule cells was monitored continuously by the cellular incorporation of [methyl-14C] thymidine using scintillating microplates. The radioisotope had no effect on cell proliferation over a 5 day period, neither was it extensively converted to thymine. Leibovitz L-15 medium supplemented with bicarbonate proved a good growth medium and its high levels of carbohydrates and amino acids facilitated the appearance of intermediates in the cells' metabolism of additional radioactive amino acids. Kidney proximal tubule cells had a greater potential to process amino acids than BHK-21 cells. The utilization of amino acids by proximal tubule cells differed from that of other organs. The amino acids could be classified into three classes. Members of the first type were only used for protein synthesis (arginine, lysine, histidine and tyrosine). The second class of amino acids yielded only one or two metabolites (leucine and isoleucine), while the last type gave more than two metabolites (alanine, aspartate, glycine, methionine, proline and valine).

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Uncontrolled Keywords: Amino acid utilization; proximal renal tubule cells; growth.
Publisher: Wiley
ISSN: 1065-6995
Last Modified: 28 Jun 2019 02:01

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