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The 5′-upstream region of human programmed cell death 5 gene contains a highly active TATA-less promoter that is up-regulated by etoposide

Xu, Mingxu, Cheng, Ning, Gui, Liming, Lai, Mouyi, Wang, Ying, Xia, Donglan, Rui, Min, Zhang, Yingmei and Ma, Dalong 2004. The 5′-upstream region of human programmed cell death 5 gene contains a highly active TATA-less promoter that is up-regulated by etoposide. Gene 329 , pp. 39-49. 10.1016/j.gene.2003.12.025

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Abstract

The PDCD5 (programmed cell death 5), a novel apoptosis related gene, is functionally associated with cell apoptosis, exhibits a ubiquitous expression pattern and is up-regulated in some types of tumor cells undergoing apoptosis. To study the transcriptional regulation of the PDCD5 gene, we have cloned 1.1 kb of its 5′-upstream region. The DNA sequencing analysis revealed a major transcriptional start site at 72 base pairs in front of the ATG translational start codon. The upstream of the transcriptional start site lacks a canonical TATA box and CAAT box. Transient transfection and luciferase assay demonstrate that this region presents extremely strong promoter activity. The 5′-deleted sequences fused to a luciferase reporter gene demonstrated that the −555/−383 region from the transcription start site is crucial for transcriptional regulation, and the luciferase reporter gene's expression significantly increased in the early stage of cell apoptosis induced by etoposide. These results imply that the PDCD5 gene may be a target gene under the control of some important apoptosis-related transcriptional factors during the cell apoptosis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
European Cancer Stem Cell Research Institute (ECSCRI)
Publisher: Elsevier
ISSN: 0378-1119
Last Modified: 04 Jun 2017 06:39
URI: http://orca.cf.ac.uk/id/eprint/63364

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